Composite

Part:BBa_K3251001:Design

Designed by: Rachel Lee   Group: iGEM19_Michigan   (2019-10-14)


IPTG-induced LuxR expression with chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2328
    Illegal AgeI site found at 2440
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1710


Design Notes

We added unique restriction sites around the promoter and the chromoproteins for easy replacement by other teams.


Source

The LuxR gene is from Vibrio fisheri

References